Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme.
Alexa GuglielmelliPaolo RosaMarco ContardiMirko PratoGiorgio ManginoSelenia MigliettaVincenzo PetrozzaRoberto PaniAntonella CalogeroAthanassia AthanassiouGiovanni PerottoLuciano De SioPublished in: Nanomedicine (London, England) (2021)
Aim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV-Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases.
Keyphrases
- electron microscopy
- high resolution
- drug release
- photodynamic therapy
- cancer therapy
- single molecule
- highly efficient
- gold nanoparticles
- drug delivery
- endothelial cells
- atomic force microscopy
- high throughput
- mass spectrometry
- small molecule
- high speed
- physical activity
- single cell
- optical coherence tomography
- magnetic resonance imaging
- stem cells
- tissue engineering
- mesenchymal stem cells
- magnetic resonance
- smoking cessation
- energy transfer
- pluripotent stem cells
- ionic liquid