Mesenchymal Stromal Cells from Healthy and Inflamed Human Gingiva Respond Differently to Porphyromonas gingivalis .
Marina BekićMarina RadanovićJelena ĐokićSergej TomićMile ErakovićDušan RadojevićMiloš DukaDejan MarkovićMilan MarkovićBashkim IsmailiDejan BokonjićMiodrag ČolićPublished in: International journal of molecular sciences (2022)
Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis , one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO-α, RANTES, TLR-2, HIF-1α, OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO-α were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF-β, LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment.
Keyphrases
- poor prognosis
- toll like receptor
- bone marrow
- cell proliferation
- inflammatory response
- mesenchymal stem cells
- immune response
- endothelial cells
- stem cells
- transcription factor
- magnetic resonance
- signaling pathway
- type diabetes
- blood brain barrier
- single cell
- gene expression
- nuclear factor
- human health
- adipose tissue
- cell therapy
- risk assessment
- long non coding rna
- computed tomography
- cell cycle
- replacement therapy