Olfml3 is a microglia-specific protein whose role in neuroinflammation is elusive. In silico analysis was conducted to characterize the Olfml3 protein, followed by molecular docking and MD simulation to check possible interaction with Iba1. Further, expression and co-localization analysis was performed in the LPS-induced neuroinflammatory mice brains. Results suggest that Olfml3 physically interacts with Iba1. Olfml3 and Iba1 expression increases during neuroinflammation in mice brains. Olfml3 was observed to co-localize with Iba1, and the number of Olfml3 and Iba1 dual-positive cells increased in the brain of the neuroinflammatory mice model. Thus, Olfml3 could potentially participate in microglia functions by interacting with Iba1.
Keyphrases
- lps induced
- molecular docking
- inflammatory response
- poor prognosis
- high fat diet induced
- binding protein
- cerebral ischemia
- lipopolysaccharide induced
- induced apoptosis
- wild type
- molecular dynamics simulations
- oxidative stress
- type diabetes
- neuropathic pain
- resting state
- long non coding rna
- amino acid
- protein protein
- molecular dynamics
- spinal cord injury
- skeletal muscle
- brain injury
- cell death
- endoplasmic reticulum stress
- virtual reality