Whole-genome sequencing reveals highly specific gene targeting by in vitro assembled Cas9-ribonucleoprotein complexes in Aspergillus fumigatus.

Qusai Al AbdallahAna Camila Oliveira SouzaAdela Martin-VicenteWenbo GeJarrod R Fortwendel

Published in: Fungal biology and biotechnology (2018)

CRISPR/Cas9-based genome editing in A. fumigatus using in vitro assembled RNPs coupled with microhomology templates is a reliable method of gene targeting. This system is highly efficient and is not associated with increased off-target mutations caused by introduction of the Cas9 nuclease.

Keyphrases

genome editing
crispr cas
highly efficient
copy number
genome wide
cancer therapy
genome wide identification
drug delivery
dna binding
genome wide analysis