Cytotoxic Properties of C 17 Polyacetylenes from the Fresh Roots of Panax ginseng on Human Epithelial Ovarian Cancer Cells.

Although C 17 polyacetylenes from Panax ginseng exhibit cytotoxic properties against various tumor cells, there have been few experiments on epithelial ovarian carcinoma cells. This study aimed to investigate the cytotoxic effects of C 17 polyacetylenes from P. ginseng against ovarian cancer cell lines. Four unreported ( 1 - 4 ) and fifteen known ( 5 - 19 ) C 17 polyacetylenes were obtained from the roots of P . ginseng using repeated chromatography (open column, MPLC, and preparative HPLC). The chemical structures of all the compounds were determined by analyzing their spectroscopic data (NMR, IR, and optical rotation) and HR-MS. The structures of new polyacetylenes were elucidated as (3 S ,8 S ,9 R ,10 R )-(-)-heptadeca-9,10-epoxy-4,6-diyne-3,8-diyl diacetate ( 1 ), (3 S ,8 S ,9 R ,10 R )-(-)-heptadeca-1-en-9,10-epoxy-4,6-diyne-3,8-diyl diacetate ( 2 ), (-)-haptadeca-9,10-epoxy-8-methoxy-4,6-diyne-3,11-diol ( 3 ), and (3 R ,9 R ,10 R )-(+)-3-acetoxy-9,10-dihydroxyheptadeca-1-en-4,6-diyne ( 4 ), named ginsenoynes O, P, and Q, and 3-acetyl panaxytriol, respectively. Subsequently, in vitro experiments on A2780 and SKOV3 human epithelial ovarian carcinoma cells were performed to assess the cytotoxic properties of the isolates. Among the isolates, panaquinquecol 4 ( 15 ) exhibited the most remarkable cytotoxic effects on both human ovarian cancer cells A2780 (IC 50 value of 7.60 μM) and SKOV3 (IC 50 value of 27.53 μM). Therefore, C 17 polyacetylenes derived from P . ginseng may warrant further investigation for their therapeutic potential in epithelial ovarian cancer.