Investigating the Human Intestinal DNA Virome and Predicting Disease-Associated Virus-Host Interactions in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS).
Shen-Yuan HsiehGeorge M SavvaAndrea TelatinSumeet K TiwariMohammad A TariqFiona NewberryKatharine A SetonCatherine BoothAmolak S BansalThomas WilemanEvelien M AdriaenssensSimon R CardingPublished in: International journal of molecular sciences (2023)
Understanding how the human virome, and which of its constituents, contributes to health or disease states is reliant on obtaining comprehensive virome profiles. By combining DNA viromes from isolated virus-like particles (VLPs) and whole metagenomes from the same faecal sample of a small cohort of healthy individuals and patients with severe myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), we have obtained a more inclusive profile of the human intestinal DNA virome. Key features are the identification of a core virome comprising tailed phages of the class Caudoviricetes , and a greater diversity of DNA viruses including extracellular phages and integrated prophages. Using an in silico approach, we predicted interactions between members of the Anaerotruncus genus and unique viruses present in ME/CFS microbiomes. This study therefore provides a framework and rationale for studies of larger cohorts of patients to further investigate disease-associated interactions between the intestinal virome and the bacteriome.
Keyphrases
- endothelial cells
- circulating tumor
- cell free
- single molecule
- induced pluripotent stem cells
- end stage renal disease
- healthcare
- public health
- pluripotent stem cells
- ejection fraction
- chronic kidney disease
- clinical trial
- nucleic acid
- newly diagnosed
- depressive symptoms
- sleep quality
- prognostic factors
- physical activity
- risk assessment
- peritoneal dialysis
- social media
- molecular dynamics simulations
- health information
- climate change