Nocardia seriolae cell wall lipids: An effective protective mechanism in resistance and virulence.
Sandra Celenia NazarethShreesha RaoLi-Wu ChengPei-Chi WangShih-Chu ChenPublished in: Journal of fish diseases (2023)
Piscine nocardiosis, caused by Nocardia seriolae, is a refractory granulomatous disease in South-East Asian aquaculture. This study investigates the virulence of nocardial lipids essential for pathogenesis among Actinomycetes. Petroleum ether (PE) was used to selectively delipidate two groups of N. seriolae, namely, live cell (LC) and killed cell (KC); resulting in delipidated live cell (DLC) and delipidated killed cell (DKC), respectively. Changes post-delipidation on genus characteristics, such as loss in acid-fast nature and resistance to lysozyme were observed. Transmission electron microscopy revealed notable changes in the lipid layer. Additionally, Lates calcarifer, Asian seabass intraperitoneally injected with LC and DLC had mortality rates of 90% and 50%, respectively, with the latter exhibiting a delay in mortality. Reverse-transcription quantitative PCR (RT-qPCR) analysis of host cytokines from the spleen and head kidney showed delipidation contributed to the induction of an immune response with increased transcriptional levels of interferon-γ (ifn-γ). Histopathological samples collected on day 7 post-inoculation displayed a varied granulomatous response between the treatment groups and scored for pathological changes. These findings affirm that the virulence of the lipids remains independent of the living state of the cell, significantly altering the immune and granulomatous responses in L. calcarifer to N. seriolae.
Keyphrases
- single cell
- immune response
- pseudomonas aeruginosa
- escherichia coli
- staphylococcus aureus
- cell therapy
- biofilm formation
- dendritic cells
- interstitial lung disease
- fatty acid
- cardiovascular events
- electron microscopy
- transcription factor
- gene expression
- mass spectrometry
- mesenchymal stem cells
- simultaneous determination
- type diabetes
- stem cells
- cardiovascular disease
- coronary artery disease
- high resolution
- toll like receptor
- bone marrow
- rheumatoid arthritis
- optical coherence tomography
- cystic fibrosis
- heat shock protein
- replacement therapy
- heat stress