Mass Spectrometry-Based Proteome Profiling of Extracellular Vesicles Derived from the Cerebrospinal Fluid of Adult Rhesus Monkeys Exposed to Cocaine throughout Gestation.
Hilal Ahmad RatherShalini MishraYixin SuAshish KumarSangeeta SinghBiswapriya Biswavas MisraJingyun LeeCristina M FurduiLindsey R HamiltonRobert W GouldSusan H NaderMichael A NaderGagan DeepPublished in: Biomolecules (2022)
Cocaine use disorder has been reported to cause transgenerational effects. However, due to the lack of standardized biomarkers, the effects of cocaine use during pregnancy on postnatal development and long-term neurobiological and behavioral outcomes have not been investigated thoroughly. Therefore, in this study, we examined extracellular vesicles (EVs) in adult (~12 years old) female and male rhesus monkeys prenatally exposed to cocaine ( n = 11) and controls ( n = 9). EVs were isolated from the cerebrospinal fluid (CSF) and characterized for the surface expression of specific tetraspanins, concentration (particles/mL), size distribution, and cargo proteins by mass spectrometry (MS). Transmission electron microscopy following immunogold labeling for tetraspanins (CD63, CD9, and CD81) confirmed the successful isolation of EVs. Nanoparticle tracking analyses showed that the majority of the particles were <200 nm in size, suggesting an enrichment for small EVs (sEV). Interestingly, the prenatally cocaine-exposed group showed ~54% less EV concentration in CSF compared to the control group. For each group, MS analyses identified a number of proteins loaded in CSF-EVs, many of which are commonly listed in the ExoCarta database. Ingenuity pathway analysis (IPA) demonstrated the association of cargo EV proteins with canonical pathways, diseases and disorders, upstream regulators, and top enriched network. Lastly, significantly altered proteins between groups were similarly characterized by IPA, suggesting that prenatal cocaine exposure could be potentially associated with long-term neuroinflammation and risk for neurodegenerative diseases. Overall, these results indicate that CSF-EVs could potentially serve as biomarkers to assess the transgenerational adverse effects due to prenatal cocaine exposure.
Keyphrases
- cerebrospinal fluid
- mass spectrometry
- prefrontal cortex
- liquid chromatography
- multiple sclerosis
- poor prognosis
- pregnant women
- preterm infants
- high resolution
- ms ms
- traumatic brain injury
- gas chromatography
- transcription factor
- photodynamic therapy
- skeletal muscle
- single cell
- adipose tissue
- insulin resistance
- lipopolysaccharide induced
- cancer therapy
- inflammatory response
- weight loss
- subarachnoid hemorrhage
- binding protein
- wound healing
- genome wide analysis