Investigation of Human and Animal Viruses in Water Matrices from a Rural Area in Southeastern Region of Brazil and Their Potential Use as Microbial Source-Tracking Markers.
Debora Regina Lopes Dos SantosMarcelle Silva-SalesTulio Machado FumianAdriana Gonçalves MaranhãoFábio Correia MaltaFernando César FerreiraMarcia Maria PimentaMarize Pereira MiagostovichPublished in: Food and environmental virology (2023)
This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specific virus (human adenovirus [HAdV], porcine adenovirus [PAdV] and bovine polyomaviruses [BoPyV]) as potential microbial source-tracking tool, and rotavirus A [RVA], given its epidemiological importance in Brazil. From July 2017 to June 2018, 92 samples were collected from eight points (P1-P8) of surface and raw waters in southeastern region of Brazil. Fifty-five (59.8%) were positive for HAdV, 41 (44.5%) for RVA, 10 (10.9%) for PAdV and four (4.3%) for BoPyV. HAdV and RVA were detected at all sites, and over the entire sampling period, PAdV was detected at a porcine breeding area and at Guarda River site, presenting high concentrations up to 2.6 × 10 9 genome copies per liter [GC/L], and viral concentrations ranging from 9.6 × 10 1 to 7.1 × 10 7 , while BoPyV (1.5 × 10 4 GC/L-9.2 × 10 5 GC/L) was only detected in samples from the bovine breeding areas. The combination of human and animal virus circulation presents a potential impact in the environment due to raw sewage discharge from regional communities, as well as potential hazard to human and animal health.
Keyphrases
- endothelial cells
- induced pluripotent stem cells
- human health
- pluripotent stem cells
- healthcare
- south africa
- public health
- mental health
- sars cov
- drinking water
- risk assessment
- microbial community
- gene expression
- mass spectrometry
- heavy metals
- case report
- gene therapy
- health risk
- simultaneous determination
- real time pcr
- antibiotic resistance genes