Low Expression of LEFTY1 in Placental Villi Is Associated with Early Unexplained Miscarriage.
Huiqin XueLan MaJinjie XueXiayu SunMing LiHongyong LuYan ZhouYuezhen GuoYuping ZhangJigeng BaiPublished in: The Journal of reproductive medicine (2018)
OBJECTIVE: To investigate the function and underlying mechanism of transforming growth factor–beta (TGF-β)/bone morphogenetic protein (BMP) signaling pathway in early unexplained miscarriage.
STUDY DESIGN: Expression profiles of genes involved in TGF-β/BMP signaling pathway were compared between placental villous tissue samples from 2 women with missed abortion and those from 2 women with induced abortion by microarray assay. The protein expression level of the most downregulated gene—LEFTY1—was further measured using western blotting in another 8 women with missed abortion and 7 women with induced abortion.
RESULTS: A total of 24 genes showed differential expression level between the 2 groups. Their functions were further investigated, of which 6 of 13 upregulated genes were TGF-β responsive genes. The most reduced gene is LEFTY1, an antagonist of TGF-β ligand. The protein expression level of LEFTY1 was confirmed to show the same trend as microarray using western blotting.
CONCLUSION: A reduced expression of LEFTY1 in women with missed abortion was identified as com-pared with women with induced abortion, which may result in a dysregulation of TGF-β signaling and may be the underlying mechanism of missed abortion.
Keyphrases
- transforming growth factor
- epithelial mesenchymal transition
- signaling pathway
- genome wide
- high glucose
- genome wide identification
- diabetic rats
- poor prognosis
- drug induced
- mesenchymal stem cells
- south africa
- pi k akt
- genome wide analysis
- endothelial cells
- oxidative stress
- induced apoptosis
- drug delivery
- endoplasmic reticulum stress
- high resolution
- transcription factor
- single cell
- cell proliferation
- bone regeneration
- single molecule
- atomic force microscopy