The Cell Type-Specific 5hmC Landscape and Dynamics of Healthy Human Hematopoiesis and TET2-Mutant Preleukemia.

Yusuke Nakauchi Armon Azizi Daniel Thomas M Ryan Corces Andreas ReinischRajiv SharmaDavid Cruz HernandezThomas KöhnkeDaiki KariganeAmy C Fan Daniel Martinez-KramsMelissa StaffordSatinder KaurRitika DuttaPaul PhanAsiri Saumya EdiriwickremaErin McCarthyYuhong NingTierney PhillipsChristopher K EllisonGulfem D GulerAnna BergamaschiChin-Jen KuSamuel Levy Ravindra Majeti

Published in: Blood cancer discovery (2022)

We show that 5-hydroxymethylation profiles are cell type-specific and associated with transcriptional abundance and chromatin accessibility across human hematopoiesis. TET2 loss caused aberrant growth and differentiation phenotypes and disrupted 5hmC and transcriptional landscapes. Treatment of TET2 KO HSPCs with ascorbate or azacitidine reverted 5hmC profiles and restored aberrant phenotypes. This article is highlighted in the In This Issue feature, p. 265.

Keyphrases

endothelial cells
gene expression
transcription factor
induced pluripotent stem cells
pluripotent stem cells
machine learning
dna damage
dna methylation
single cell
oxidative stress