Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis.
Bram GerritsenAridaman PanditFatiha Zaaraoui-BoutaharMirjam C G N van den HoutWilfred F J Van IJckenRob J De BoerArno C AndewegPublished in: Immunogenetics (2019)
The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, J, and C genes. The ferret locus appears to be most similar to that of the dog. Like other mammalian TRB loci, the ferret TRB locus contains a library of variable genes located upstream of two D-J-C gene clusters, followed by a (in the ferret non-functional) V gene with an inverted transcriptional orientation. All TRB genes (expressed or not) reported here have been approved by the IMGT/WHO-IUIS nomenclature committee.
Keyphrases
- genome wide identification
- genome wide
- transcription factor
- copy number
- dna methylation
- peripheral blood
- genome wide analysis
- genome wide association study
- endothelial cells
- bioinformatics analysis
- high throughput sequencing
- gene expression
- systematic review
- young adults
- randomized controlled trial
- deep learning
- induced pluripotent stem cells
- heat shock protein