Light-induced Nrf2-/- mice as atrophic age-related macular degeneration model and treatment with nanoceria laden injectable hydrogel.
Kai WangMin ZhengKaitlyn Lee LesterZongchao HanPublished in: Scientific reports (2019)
Elevated oxidative stress and associated reactive oxygen species (ROS) accumulation are hallmarks in the induction and progression of age-related macular degeneration (AMD). By exposing nuclear factor erythroid 2-related factor (Nrf2) knockout (Nrf2-/-) mice to mild white light, we were able to generate a new dry-AMD like murine model to the study. This animal model developed phenotypes of photoreceptor degeneration, retinal function impairment, ROS accumulation, and inflammation reaction in a relatively shorter time. In the treatment of this animal model we utilized an antioxidative and water soluble nanoparticle known as glycol chitosan coated cerium oxide nanoparticles (GCCNP). The delivery of GCCNP protected retina against progressive retinal oxidative damage. Further combination of GCCNP with alginate-gelatin based injectable hydrogel provided synergistic antioxidant effects and achieved a more rapid recovery of the retinal pigment epithelium and photoreceptor cells. This combined treatment technique has the potential to translate into a clinical intervention for the treatment of AMD.
Keyphrases
- oxidative stress
- age related macular degeneration
- reactive oxygen species
- drug delivery
- randomized controlled trial
- nuclear factor
- dna damage
- hyaluronic acid
- induced apoptosis
- toll like receptor
- adipose tissue
- metabolic syndrome
- multiple sclerosis
- cell death
- tissue engineering
- cell proliferation
- type diabetes
- risk assessment
- inflammatory response
- oxide nanoparticles
- ischemia reperfusion injury
- climate change
- human health
- sensitive detection