Transendothelial Migration of Human B Cells: Chemokine versus Antigen.
Vivian WangJordan S PoberThomas D ManesPublished in: Journal of immunology (Baltimore, Md. : 1950) (2023)
B cells, like T cells, can infiltrate sites of inflammation, but the processes and B cell subsets involved are poorly understood. Using human cells and in vitro assays, we find only a very small number of B cells will adhere to TNF-activated (but not to resting) human microvascular endothelial cells (ECs) under conditions of venular flow and do so by binding to ICAM-1 and VCAM-1. CXCL13 and, to a lesser extent, CXCL10 bound to the ECs can increase adhesion and induce transendothelial migration (TEM) of adherent naive and memory B cells in 10-15 min through a process involving cell spreading, translocation of the microtubule organizing center (MTOC) into a trailing uropod, and interacting with EC activated leukocyte cell adhesion molecule. Engagement of the BCR by EC-bound anti-κ L chain Ab also increases adhesion and TEM of κ+ but not λ+ B cells. BCR-induced TEM takes 30-60 min, requires Syk activation, is initiated by B cell rounding up and translocation of the microtubule organizing center to the region of the B cell adjacent to the EC, and also uses EC activated leukocyte cell adhesion molecule for TEM. BCR engagement reduces the number of B cells responding to chemokines and preferentially stimulates TEM of CD27+ B cells that coexpress IgD, with or without IgM, as well as CD43. RNA-sequencing analysis suggests that peripheral blood CD19+CD27+CD43+IgD+ cells have increased expression of genes that support BCR activation as well as innate immune properties in comparison with total peripheral blood CD19+ cells.
Keyphrases
- cell adhesion
- peripheral blood
- endothelial cells
- acute lymphoblastic leukemia
- tyrosine kinase
- high glucose
- induced apoptosis
- chronic myeloid leukemia
- cell cycle arrest
- innate immune
- oxidative stress
- social media
- nk cells
- poor prognosis
- induced pluripotent stem cells
- stem cells
- biofilm formation
- working memory
- hiv infected
- blood pressure
- cell death
- genome wide
- signaling pathway
- staphylococcus aureus
- heart rate variability
- diabetic rats
- pseudomonas aeruginosa