Application of Direct Sonoporation from a Defined Surface Area of the Peritoneum: Evaluation of Transfection Characteristics in Mice.
Koyo NishimuraKeita YonezawaShintaro FumotoYusuke MiuraMasayori HagimoriKoyo NishidaShigeru KawakamiPublished in: Pharmaceutics (2019)
In the present study, we developed a sonoporation system, namely "direct sonoporation", for transfecting the peritoneum from a defined surface area to avoid systematic side effects. Here, the transfection characteristics are explained because there is less information about direct sonoporation. Naked pDNA and nanobubbles were administered to diffusion cell attached to the visceral and parietal peritoneum from the liver and peritoneal wall surface, respectively. Then, ultrasound was irradiated. Direct sonoporation showed a higher transfection efficacy at the applied peritoneum site from the liver surface while other sites were not detected. Moreover, transgene expression was observed in the peritoneal mesothelial cells (PMCs) at the applied peritoneum site. No abnormality was observed in the inner part of the liver. Although transgene expression of the visceral peritoneum was tenfold higher than that of the parietal peritoneum, transgene expression was observed in the PMCs on both the applied peritoneum sites. These results suggest that direct sonoporation is a site-specific transfection method of the PMCs on the applied peritoneum site without transgene expression at other sites and show little toxicity in the inner tissues at the applied site via cavitation energy. This information is valuable for the development of an intraperitoneal sonoporation device for treatment of peritoneal diseases such as peritoneal fibrosis.
Keyphrases
- poor prognosis
- binding protein
- magnetic resonance imaging
- gene expression
- working memory
- insulin resistance
- long non coding rna
- oxidative stress
- induced apoptosis
- adipose tissue
- type diabetes
- single cell
- computed tomography
- health information
- metabolic syndrome
- skeletal muscle
- ultrasound guided
- endothelial cells
- liver fibrosis