MicroRNA-184 promotes apoptosis of trophoblast cells via targeting WIG1 and induces early spontaneous abortion.
Yuan ZhangJi ZhouMing-Qing LiJie XuJin-Ping ZhangLi-Ping JinPublished in: Cell death & disease (2019)
Recurrent spontaneous abortion (RSA) refers to the unintentional termination of two or more consecutive pregnancies that severely threatens human reproductive health. Our previous study has shown that miR-184 is expressed more highly in RSA than in normal pregnancy, whether in the villus or decidua. In this study, compared with normal pregnant women, the expression of miR-184 in decidual stromal cells (DSCs) and decidual immune cells (DICs), as well as in peripheral blood, from RSA patients was enhanced similarly. Moreover, we found miR-184 could promote the apoptosis and repress the proliferation of trophoblast cells. Further exploration indicated that miR-184 upregulated the expression of Fas by targeting WIG1 thus inducing cell apoptosis. Finally, after miR-184 overexpression in vivo, the embryo resorption rate in pregnant mice was increased significantly. Therefore, our study outlines the pivotal role of miR-184 in maintaining successful pregnancy, providing a new diagnostic and therapeutic target for RSA.
Keyphrases
- cell proliferation
- long non coding rna
- long noncoding rna
- cell cycle arrest
- pregnant women
- poor prognosis
- induced apoptosis
- peripheral blood
- end stage renal disease
- oxidative stress
- endoplasmic reticulum stress
- cell death
- preterm birth
- signaling pathway
- pregnancy outcomes
- pi k akt
- type diabetes
- metabolic syndrome
- ejection fraction
- peritoneal dialysis
- adipose tissue
- drug delivery
- transcription factor
- insulin resistance
- skeletal muscle
- binding protein
- wild type