PROTAC-mediated vimentin degradation promotes terminal erythroid differentiation of pluripotent stem cells.

Hao YanRuge ZangTiantian CuiYiming LiuBiao ZhangLingpin ZhaoHongyu LiJuannian ZhouHaiyang WangQuan ZengLei XuYuqi ZhouXuetao PeiJiafei XiWen Yue

Published in: Stem cell research & therapy (2024)

VIM disappear during the normal maturation of erythroid cells, whereas they are retained in erythroid cells differentiated from hPSCs. We found that retention of vimentin during erythropoiesis impairs erythroid enucleation from hPSCs. Using the PROTAC platform, we validated that vimentin degradation by dTAG accelerates the enucleation rate in dTAG-VIM-H9 cells by enhancing nuclear polarization.

Keyphrases

induced apoptosis
cell cycle arrest
endoplasmic reticulum stress
signaling pathway
cell death
cell proliferation
high throughput
pluripotent stem cells