Effects and Eradication of Mycoplasma Contamination on Patient-derived Colorectal Cancer Organoid Cultures.
Divya L DayanidhiWylie K WatlingtonJohn B MantyhGabrielle RupprechtShiao-Wen D HsuPublished in: Cancer research communications (2023)
Organoids are valuable models of cancer. Mycoplasma contamination can alter organoid drug sensitivity, so there is a need for a standardized protocol to detect and remove Mycoplasma from organoids. We report a simple procedure for removing Mycoplasma from organoids via in vivo passaging through mice followed by re-establishment of organoids.
Keyphrases
- respiratory tract
- risk assessment
- induced pluripotent stem cells
- drinking water
- health risk
- randomized controlled trial
- papillary thyroid
- human health
- minimally invasive
- type diabetes
- squamous cell carcinoma
- emergency department
- helicobacter pylori infection
- adipose tissue
- squamous cell
- young adults
- high fat diet induced
- lymph node metastasis