Biodegradation of Diesel, Crude Oil and Spent Lubricating Oil by Soil Isolates of Bacillus spp.
Maddela Naga RajuRodriguez LeoSanaguano Salguero HerminiaRicardo Ernesto Burgos MoránKadiyala VenkateswarluScalvenzi LauraPublished in: Bulletin of environmental contamination and toxicology (2017)
Two species of Bacillus, B. thuringiensis B3 and B. cereus B6, isolated from crude oil-contaminated sites in Ecuador, were tested for their capability in degrading polycyclic aromatic hydrocarbons (PAHs) in diesel (shake-flask), and to remove total petroleum hydrocarbons (TPHs) from crude oil- or spent lubricating oil-polluted soils (plot-scale). TPHs and PAHs were analyzed by Gas chromatography-Flame ionization detector (GC-FID) and High performance liquid chromatography (HPLC), respectively. Degradation percentages of PAHs by strain B6 were in the range of 11-83 after 30 days. A mixed culture of both the strains removed 84% and 28% of TPHs from crude oil- and spent lubricating oil-polluted soils, respectively. Reduction in the abundance of total n-alkane fractions (C8-C40) of spent lubricating oil was 94%, which was 18% higher than the control. Our results clearly indicate that the selected strains have great potential in degrading petroleum hydrocarbons at both laboratory- and field-scales.
Keyphrases
- heavy metals
- polycyclic aromatic hydrocarbons
- gas chromatography
- high performance liquid chromatography
- fatty acid
- tandem mass spectrometry
- mass spectrometry
- human health
- health risk assessment
- risk assessment
- solid phase extraction
- simultaneous determination
- gas chromatography mass spectrometry
- bacillus subtilis