Login / Signup

Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.

Tingting DingChaoyong HuangZeyu LiangXiaoyan MaNing WangYi-Xin Huo
Published in: Microbial cell factories (2020)
The RPGPs cloning strategy serves as a general solution to avoid plasmid RecA-independent recombination. It can be adapted to applications that rely on paired gRNAs or repeated genetic parts.
Keyphrases
  • crispr cas
  • genome editing
  • escherichia coli
  • dna damage
  • genome wide
  • dna repair
  • biofilm formation
  • klebsiella pneumoniae
  • copy number
  • dna methylation
  • multidrug resistant