Reversed paired-gRNA plasmid cloning strategy for efficient genome editing in Escherichia coli.
Tingting DingChaoyong HuangZeyu LiangXiaoyan MaNing WangYi-Xin HuoPublished in: Microbial cell factories (2020)
The RPGPs cloning strategy serves as a general solution to avoid plasmid RecA-independent recombination. It can be adapted to applications that rely on paired gRNAs or repeated genetic parts.