Roundabout homolog 1 inhibits proliferation via the YY1-ROBO1-CCNA2-CDK2 axis in human pancreatic cancer.
Kui-Rong JiangPeng ShenWan-Li GeTao-Yue YangWu-Jun WangLing-Dong MengXu-Min HuangYi-Han ZhangShou-Ji CaoYi MiaoKui-Rong JiangJing-Jing ZhangPublished in: Oncogene (2021)
Pancreatic cancer (PC) is highly malignant and has a high mortality with a 5-year survival rate of less than 8%. As a member of the roundabout immunoglobulin superfamily of proteins, ROBO1 plays an important role in embryogenesis and organogenesis and also inhibits metastasis in PC. Our study was designed to explore whether ROBO1 has effects on the proliferation of PC and its specific mechanism. The expression of ROBO1 was higher in cancer tissues than in matched adjacent tissues by immunohistochemistry (IHC) and qRT-PCR. Low ROBO1 expression is associated with PC progression and poor prognosis. Overexpression of ROBO1 can inhibit the proliferation of PC cells in vitro, and the S phase fraction can also be induced. Further subcutaneous tumor formation in nude mice showed that ROBO1 overexpression can significantly inhibit tumor growth. YY1 was found to directly bind to the promoter region of ROBO1 to promote transcription by a luciferase reporter gene assay, a chromatin immunoprecipitation (ChIP) and an electrophoretic mobility shift assay (EMSA). Mechanistic studies showed that YY1 can inhibit the development of PC by directly regulating ROBO1 via the CCNA2/CDK2 axis. Taken together, our results suggest that ROBO1 may be involved in the development and progression of PC by regulating cell proliferation and shows that ROBO1 may be a novel and promising therapeutic target for PC.
Keyphrases
- poor prognosis
- cell proliferation
- gene expression
- transcription factor
- long non coding rna
- high throughput
- signaling pathway
- dna methylation
- endothelial cells
- metabolic syndrome
- squamous cell carcinoma
- genome wide
- cell cycle
- cardiovascular disease
- coronary artery disease
- dna damage
- risk factors
- oxidative stress
- cardiovascular events
- papillary thyroid
- high glucose
- copy number
- pi k akt
- diabetic rats
- high throughput sequencing