An adapted protocol to derive microglia from stem cells and its application in the study of CSF1R-related disorders.

Marie-France DorionDiana CasasIrina ShlaiferMoein YaqubiPeter FlemingNathan KarpilovskyCarol X-Q ChenMichael NicouleauValerio E C PiscopoEmma J MacDougallAeshah AlluliTaylor M GoldsmithAlexandria SchneiderSamuel DorionNathalia AprahamianAdam MacDonaldRhalena A ThomasRoy W R DudleyJeffrey A HallEdward A FonJack P AntelJo Anne StrattonThomas M DurcanRoberta La PianaLuke Michael Healy

Published in: Molecular neurodegeneration (2024)

We optimized a pre-existing iMGL protocol, generating a powerful tool to study microglial involvement in human neurological diseases. Using the optimized protocol, we have generated for the first time iMGL from an ALSP patient carrying a pathogenic CSF1R variant, with preliminary characterization pointing toward functional alterations in migratory, phagocytic and inflammatory activities.

Keyphrases

stem cells
randomized controlled trial
inflammatory response
endothelial cells
oxidative stress
neuropathic pain
case report
lipopolysaccharide induced
mesenchymal stem cells
brain injury
spinal cord
cerebrospinal fluid
cell therapy
induced pluripotent stem cells
cerebral ischemia