Login / Signup

Time-resolved cryo-EM using Spotiton.

Venkata P DandeyWilliam C BudellHui WeiDaija BobeKashyap MaruthiMykhailo KopylovEdward T EngPeter A KahnJenny E HinshawNidhi KunduCrina M NimigeanChen FanNattakan SukomonSeth A DarstRuth M SaeckerJames ChenBrandon MaloneClinton S PotterBridget Carragher
Published in: Nature methods (2020)
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.
Keyphrases
  • electron microscopy
  • mass spectrometry
  • multiple sclerosis
  • ms ms
  • high resolution
  • room temperature
  • ionic liquid