Analytical validation of a standardised scoring protocol for Ki67 immunohistochemistry on breast cancer excision whole sections: an international multicentre collaboration.
Samuel C Y LeungTorsten O NielsenLila A ZabagloIndu ArunSunil S BadveAnita L BaneJohn M S BartlettSigne BorgquistMartin C ChangAndrew DodsonAnna EhingerSusan FinebergCornelia M FockeDongxia GaoAllen M GownCarolina GutierrezJudith C HughZuzana KosAnne-Vibeke LaenkholmMauro G MastropasquaTakuya MoriyaSharon Nofech-MozesC Kent OsborneFrédérique M Penault-LlorcaTammy PiperTakashi SakataniRoberto SalgadoJane StarczynskiTomoharu SugieBert Van der VegtGiuseppe VialeDaniel F HayesLisa M McShaneMitch Dowsettnull nullPublished in: Histopathology (2019)
The global scoring method demonstrates adequate reproducibility to warrant next steps towards evaluation for technical and clinical validity in appropriate cohorts of cases. The time taken for scoring by either method is practical using counting software we are making publicly available. Establishment of external quality assessment schemes is likely to improve the reproducibility between laboratories further.