Association between IL-6, miRNA-146a, MALAT1 genetic polymorphisms and risk of rheumatoid arthritis.
Yasser B M AliNoura Ma HasanEman A El-MaadawyIman Hamed BassyouniMohamed El-ShahatRoba M TalaatPublished in: Personalized medicine (2024)
Aim: This study aimed to investigate the associations between single nucleotide polymorphisms (SNPs) of IL-6 (-174G/C), microRNA146a (rs2910164C/G) and MALAT1 (rs619586A/G) and susceptibility to rheumatoid arthritis (RA) in Egyptians. Methods: SNPs were genotyped in 101 RA patients and 104 controls. Expression levels were evaluated either by Enzyme-linked immunosorbent assay (ELISA) for IL-6 or quantitative real-time PCR (qRT-PCR) for miR-146a and MALAT1. Results: IL-6-174 GC (OR = 3.422) genotype, IL-6-174 C allele (OR = 2.565), miR-146a (rs2910164) CG (OR = 2.190) and MALAT1 (rs619586) AA (OR = 4.125) genotypes and A allele (OR = 6.122) could be considered as risk factors for RA. An increase in the expression of IL-6, miR-146a and MALAT1 was detected in RA patients, which was independent of any SNP. Conclusion: SNPs of IL-6, miR-146a and MALAT1were linked to RA predisposition in Egyptians.
Keyphrases
- rheumatoid arthritis
- disease activity
- long non coding rna
- cell proliferation
- end stage renal disease
- poor prognosis
- ankylosing spondylitis
- long noncoding rna
- genome wide
- ejection fraction
- newly diagnosed
- chronic kidney disease
- interstitial lung disease
- prognostic factors
- systemic lupus erythematosus
- real time pcr
- gene expression
- high throughput
- mass spectrometry
- genome wide association
- liquid chromatography