Whole Blood Dendritic Cell Cytokine Production Assay.
Tonia WoodberryJessica Rita LoughlandGabriela MinigoPublished in: Methods in molecular biology (Clifton, N.J.) (2022)
This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c + DC, CD141 + DC, and CD16 + DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.
Keyphrases
- dendritic cells
- immune response
- toll like receptor
- regulatory t cells
- healthcare
- inflammatory response
- nuclear factor
- randomized controlled trial
- endothelial cells
- induced apoptosis
- mental health
- public health
- emergency department
- oxidative stress
- high throughput
- health information
- adverse drug
- reactive oxygen species
- toxoplasma gondii
- cell cycle arrest