590 nm LED Irradiation Improved Erythema through Inhibiting Angiogenesis of Human Microvascular Endothelial Cells and Ameliorated Pigmentation in Melasma.
Xiaoxi DaiShanglin JinYijie XuanYiwen YangXiaoli LuChen WangLi ChenLeihong XiangChengfeng ZhangPublished in: Cells (2022)
Melasma is a common refractory acquired pigmentary skin disease that mainly affects middle-aged women. The pathogenesis of melasma is still uncertain, while abnormal vascular endothelial cells may play a role. We previously demonstrated the yellow light of light-emitting diodes (LED) could inhibit melanogenesis through the photobiomodulation (PBM) of melanocytes and keratinocytes. In the current study, we investigated the effect of 590 nm LED on the function of human microvascular endothelial cells (HMEC-1). We revealed 0-40 J/cm 2 590 nm LED had no toxic effect on HMEC-1 in vitro. 590 nm LED irradiation significantly reduced cell migration, tube formation, as well as the expression of vascular endothelial growth factor (VEGF) and stem cell factor (SCF), a pro-melanogenic factor. Moreover, we illustrated that 590 nm LED inhibited the phosphorylation of the AKT/PI3K/mTOR signaling pathway, and the inhibitory effect on HMEC-1 could be partially reversed by insulin-like growth factor 1 (IGF-1), an AKT/PI3K/mTOR pathway agonist. Besides, we conducted a pilot clinical study and observed a marked improvement on facial erythema and pigmentation in melasma patients after amber LED phototherapy. Taken together, 590 nm LED inhibited HMEC-1 migration, tube formation and the secretion of VEGF and SCF, predominantly through the inhibition of the AKT/PI3K/mTOR pathway, which may serve as a novel therapeutic option for melasma.
Keyphrases
- endothelial cells
- vascular endothelial growth factor
- light emitting
- signaling pathway
- high glucose
- photodynamic therapy
- cell proliferation
- stem cells
- cell migration
- poor prognosis
- ejection fraction
- adipose tissue
- newly diagnosed
- type diabetes
- end stage renal disease
- binding protein
- clinical trial
- endoplasmic reticulum stress
- bone marrow
- double blind
- mass spectrometry
- atomic force microscopy
- african american
- pregnancy outcomes
- induced pluripotent stem cells