Beneficial Effects of Linseed Supplementation on Gut Mucosa-Associated Microbiota in a Physically Active Mouse Model of Crohn's Disease.
Claire PlissonneauAdeline SivignonBenoit ChassaingFrédéric CapelVincent MartinMonique EtienneIvan WawrzyniakPierre ChausseFrederic DutheilGuillaume MairesseGuillaume ChesneauNathalie BoisseauNicolas BarnichPublished in: International journal of molecular sciences (2022)
The Western diet, rich in lipids and in n-6 polyunsaturated fatty acids (PUFAs), favors gut dysbiosis observed in Crohn's disease (CD). The aim of this study was to assess the effects of rebalancing the n-6/n-3 PUFA ratio in CEABAC10 transgenic mice that mimic CD. Mice in individual cages with running wheels were randomized in three diet groups for 12 weeks: high-fat diet (HFD), HFD + linseed oil (HFD-LS-O) and HFD + extruded linseed (HFD-LS-E). Then, they were orally challenged once with the Adherent-Invasive Escherichia coli (AIEC) LF82 pathobiont. After 12 weeks of diet, total energy intake, body composition, and intestinal permeability were not different between groups. After the AIEC-induced intestinal inflammation, fecal lipocalin-2 concentration was lower at day 6 in n-3 PUFAs supplementation groups (HFD-LS-O and HFD-LS-E) compared to HFD. Analysis of the mucosa-associated microbiota showed that the abundance of Prevotella , Paraprevotella , Ruminococcus , and Clostridiales was higher in the HFD-LS-E group. Butyrate levels were higher in the HFD-LS-E group and correlated with the Firmicutes/Proteobacteria ratio. This study demonstrates that extruded linseed supplementation had a beneficial health effect in a physically active mouse model of CD susceptibility. Additional studies are required to better decipher the matrix influence in the linseed supplementation effect.
Keyphrases
- high fat diet
- insulin resistance
- adipose tissue
- body composition
- mouse model
- escherichia coli
- physical activity
- weight loss
- oxidative stress
- randomized controlled trial
- high fat diet induced
- mental health
- metabolic syndrome
- skeletal muscle
- cystic fibrosis
- south africa
- resistance training
- social media
- health information
- placebo controlled
- drug induced
- preterm birth
- stress induced