Quantitative Assessment of Dietary (Poly)phenol Intake: A High-Throughput Targeted Metabolomics Method for Blood and Urine Samples.
Maite Domínguez-FernándezYifan XuPaul Young Tie YangWafa AlotaibiRachel GibsonWendy L HallLeon BarronIziar A LudwigConcepción CidAna Rodriguez-MateosPublished in: Journal of agricultural and food chemistry (2020)
Many studies have associated the consumption of (poly)phenol-rich diets with health benefits. However, accurate high-throughput quantitative methods for estimating exposure covering a broad spectrum of (poly)phenols are lacking. We have developed and validated a high-throughput method for the simultaneous quantification of 119 (poly)phenol metabolites in plasma and urine using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry, with a very fast sample treatment and a single run time of 16 min. This method is highly sensitive, precise, accurate, and shows good linearity for all compounds (R2 > 0.992). This novel method will allow a quantitative assessment of habitual (poly)phenol intake in large epidemiological studies as well as clinical studies investigating the health benefits of dietary (poly)phenols.
Keyphrases
- high throughput
- mass spectrometry
- tandem mass spectrometry
- high resolution
- liquid chromatography
- healthcare
- public health
- ultra high performance liquid chromatography
- single cell
- simultaneous determination
- high performance liquid chromatography
- gas chromatography
- high resolution mass spectrometry
- body mass index
- physical activity
- risk assessment
- drug delivery
- cancer therapy
- single molecule
- social media