One-tube detection of Salmonella Typhimurium using LAMP and CRISPR-Cas12b.

Jiansen Gong Yi JiangDi ZhangTingting LiLixia FuXinhong Dou

Published in: Microbiology spectrum (2024)

Typhimurium detection in one pot by integrating the LAMP assay with the CRISPR/Cas12b system, offering significant advantages in terms of simplicity, speed, and accuracy.

Keyphrases

loop mediated isothermal amplification
crispr cas
genome editing
listeria monocytogenes
sensitive detection
escherichia coli
real time pcr
high throughput
label free