Rapid Bacterial Detection and Gram-Identification Using Bacterially Activated, Macrophage-Membrane-Coated Nanowired-Si Surfaces in a Microfluidic Device.
Sidi LiuHuibo WangLe YuYijin RenHjalmar R BoumaJian LiuHenny C van der MeiHenk J BusscherPublished in: Nano letters (2023)
Bacterially induced sepsis requires rapid bacterial detection and identification. Hours count for critically ill septic patients, while current culture-based detection requires at least 10 h up to several days. Here, we apply a microfluidic device equipped with a bacterially activated, macrophage-membrane-coating on nanowired-Si adsorbent surfaces for rapid, bacterial detection and Gram-identification in bacterially contaminated blood. Perfusion of suspensions of Gram-negative or Gram-positive bacteria through a microfluidic device equipped with membrane-coated adsorbent surfaces detected low (<10 CFU/mL) bacterial levels. Subsequent, in situ fluorescence-staining yielded Gram-identification for guiding antibiotic selection. In mixed Escherichia coli and Staphylococcus aureus suspensions, Gram-negative and Gram-positive bacteria were detected in the same ratios as those fixed in suspension. Results were validated with a 100% correct score by blinded evaluation (two observers) of 15 human blood samples, spiked with widely different bacterial strains or combinations of strains, demonstrating the potential of the platform for rapid (1.5 h in total) diagnosis of bacterial sepsis.
Keyphrases
- gram negative
- loop mediated isothermal amplification
- multidrug resistant
- escherichia coli
- label free
- high throughput
- biofilm formation
- staphylococcus aureus
- sensitive detection
- acute kidney injury
- circulating tumor cells
- end stage renal disease
- intensive care unit
- adipose tissue
- endothelial cells
- klebsiella pneumoniae
- chronic kidney disease
- bioinformatics analysis
- ejection fraction
- magnetic resonance imaging
- heavy metals
- septic shock
- randomized controlled trial
- risk assessment
- peripheral blood
- cystic fibrosis
- candida albicans
- methicillin resistant staphylococcus aureus
- single molecule
- room temperature
- aqueous solution
- diabetic rats
- ionic liquid
- human health
- clinical evaluation