Recombinant mouse calcitonin gene-related peptide secreted by Lactococcus lactis inhibits lipopolysaccharide-induced inflammatory response in macrophages.
Fu NamaiYoshinari YamamotoTakashi SatoTasuku OgitaTakeshi ShimosatoPublished in: Animal science journal = Nihon chikusan Gakkaiho (2018)
We describe the development of a genetically modified strain of lactic acid bacteria (gmLAB) capable of producing a recombinant mouse calcitonin gene-related peptide (rCGRP). This strain (NZ-CGRP) was generated by introducing a CGRP secretion plasmid into Lactococcus lactis NZ9000. Western blotting confirmed the secretion of rCGRP in the presence of the inducer nisin. Highly purified rCGRP was obtained from the culture supernatants of NZ-CGRP. We demonstrated that prophylactic exposure of a culture of mouse peritoneal macrophages to rCGRP inhibited lipopolysaccharide (LPS) induction of tumor necrosis factor-α (TNF-α). The rCGRP-secreting gmLAB strain holds promise for development as a new anti-inflammatory prophylactic.
Keyphrases
- inflammatory response
- lipopolysaccharide induced
- lps induced
- lactic acid
- toll like receptor
- anti inflammatory
- rheumatoid arthritis
- copy number
- genome wide
- escherichia coli
- crispr cas
- genome wide identification
- cell free
- south africa
- machine learning
- big data
- immune response
- transcription factor
- deep learning
- dna methylation
- drug induced