Klebsiella variicola is an opportunistic pathogen often misidentified as Klebsiella pneumoniae , leading to misdiagnoses and inappropriate treatment in clinical settings. The genetic and molecular characteristics of clinically isolated K. variicola remain largely unexplored. We aim to fill this knowledge gap by examining the genomic properties of and evolutionary relationships between clinical isolates of K. variicola . The genomic data of 70 K. variicola strains were analyzed using whole-genome sequencing. A phylogenetic tree was generated based on the gene sequences from these K. variicola strains and public databases. Among the K. variicola strains, the drug resistance genes with the highest carrying rates were beta-lactamase and aminoglycoside. Locally isolated strains had a higher detection rate for virulence genes than those in public databases, with yersiniabactin genes being the most prevalent. The K locus types and MLST subtypes of the strains exhibited a dispersed distribution, with O3/O3a being the predominant subtype within the O category. In total, 28 isolates carried both IncFIB(K)_Kpn3 and IncFII_pKP91 replicons. This study underscores the importance of developing more effective diagnostic tools and therapeutic strategies for K. variicola infections. The continued surveillance and monitoring of K. variicola strains is essential for understanding the epidemiology of infections and informing public health strategies.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- genome wide
- public health
- copy number
- healthcare
- genome wide identification
- pseudomonas aeruginosa
- multidrug resistant
- mental health
- dna methylation
- biofilm formation
- emergency department
- bioinformatics analysis
- risk factors
- single cell
- electronic health record
- transcription factor
- drug resistant
- acinetobacter baumannii
- genome wide analysis
- gram negative
- adverse drug
- global health
- label free
- genome wide association study