Hydrogen Peroxide-Releasing Hydrogels for Enhanced Endothelial Cell Activities and Neovascularization.
Yunki LeeJoo Young SonJeon Il KangKyung Min ParkThai Thanh Hoang ThiPublished in: ACS applied materials & interfaces (2018)
Reactive oxygen species (ROS) have been implicated as a critical modulator for various therapeutic applications such as treatment of vascular disorders, wound healing, and cancer treatment. Specifically, growing evidence has recently demonstrated that transient or low levels of hydrogen peroxide (H2O2) facilitates tissue regeneration and wound repair through acute oxidative stress that can evaluate intracellular ROS levels in cells or tissues. Herein, we report a gelatin-based H2O2-releasing hydrogel formed by dual enzyme-mediated reaction using horseradish peroxidase and glucose oxidase (GO x). The release behavior of H2O2 from the hydrogel matrices can be precisely controlled by varying the GO x concentrations. We demonstrate that H2O2-releasing hydrogels with the optimal condition increase transient upregulation of intracellular ROS levels in the endothelial cells (ECs), enhance proliferative activities of ECs in vitro, and facilitate neovascularization in ovo. We suggest that our H2O2-releasing hydrogels hold great potential as an injectable and dynamic matrix for the treatment of vascular disorders as well as in tissue regenerative medicine.
Keyphrases
- hydrogen peroxide
- wound healing
- reactive oxygen species
- hyaluronic acid
- tissue engineering
- endothelial cells
- drug delivery
- nitric oxide
- dna damage
- oxidative stress
- induced apoptosis
- vascular endothelial growth factor
- cell death
- stem cells
- gene expression
- drug release
- metabolic syndrome
- liver failure
- diabetic retinopathy
- poor prognosis
- type diabetes
- respiratory failure
- intensive care unit
- signaling pathway
- hepatitis b virus
- combination therapy
- insulin resistance
- blood pressure
- climate change
- risk assessment
- ischemia reperfusion injury
- endoplasmic reticulum stress
- long non coding rna
- drug induced
- high glucose
- human health