Localization of PD-L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles.
Elzbieta StepulaMatthias KönigXin-Ping WangJanina LevermannTobias SchimmingSabine Kasimir-BauerBastian SchillingSebastian SchlückerPublished in: Journal of biophotonics (2020)
Programmed cell death-ligand 1 (PD-L1) is an important predictive biomarker. The detection of PD-L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno-SERS microscopy (iSERS) for localizing PD-L1 on single cancer SkBr-3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near-infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False-color iSERS images of the positive and negative controls clearly reveal the specific localization of PD-L1 with SERS nanotag-labeled antibodies.
Keyphrases
- sensitive detection
- label free
- induced apoptosis
- gold nanoparticles
- loop mediated isothermal amplification
- advanced cancer
- high speed
- reduced graphene oxide
- quantum dots
- cell cycle arrest
- palliative care
- optical coherence tomography
- high resolution
- single molecule
- raman spectroscopy
- gene expression
- oxidative stress
- energy transfer
- papillary thyroid
- squamous cell carcinoma
- mass spectrometry
- computed tomography
- machine learning
- genome wide
- lymph node metastasis