A robust method for RNA extraction and purification from a single adult mouse tendon.
Mor GrinsteinHeather L DingwallRishita R ShahTerence D CapelliniJenna L GallowayPublished in: PeerJ (2018)
Our work presents a robust, cost-effective, and straightforward method to extract high quality RNA from a single adult mouse Achilles tendon at sufficient amounts for RT-qPCR as well as RNA-seq. We show this can reduce variation and decrease the overall costs associated with experiments. This approach can also be applied to other skeletal tissues, as well as precious human samples.