Durable targeting of B-lymphocytes in living mice.
M CascalhoD HuynhA R LeffertsL SteinT LaniganJoseph T DeckerL D SheaJ L PlattPublished in: Scientific reports (2018)
Transfer to and enduring expression of genes in B cells has proved a vexing challenge. We report here a novel method for the specific and durable targeting of B lymphocytes in living mice. The method involves generation of lentiviruses pseudotyped with an anti-CD19 antibody. CD19 targeting viruses injected in the spleen of living mice efficiently transduced B cells and plasma cells detected by flow cytometry analysis of GFP expression. Expression of the reporter gene could be detected in the intact animal by external imaging for more than a year and was enhanced by booster immunization. Our method thus enables the specific delivery, expression and localization by external imaging of exogenous genes to the B cells and plasma cells of living individuals.
Keyphrases
- poor prognosis
- induced apoptosis
- flow cytometry
- high resolution
- genome wide
- binding protein
- high fat diet induced
- cancer therapy
- long non coding rna
- cell cycle arrest
- type diabetes
- gene expression
- adipose tissue
- oxidative stress
- dna methylation
- photodynamic therapy
- cell proliferation
- wild type
- fluorescence imaging
- insulin resistance
- pi k akt