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Structural Analyses of the Glycolipids in Lipid Rafts.

Yusuke SuzukiKazuya Kabayama
Published in: Methods in molecular biology (Clifton, N.J.) (2023)
Lipid rafts are usually isolated from cells or tissues using sucrose gradient ultracentrifugation in the presence of detergents such as Triton X-100 at 4 °C. Although detergents should be removed for further structural characterization following fractionation, these compounds are often difficult to completely remove, especially from the glycolipids. In this chapter, we describe a novel method for the fast and convenient removal of detergents from lipid raft glycolipids following fraction and describe the application of this method.
Keyphrases
  • fatty acid
  • induced apoptosis
  • gene expression
  • cell proliferation
  • cell death
  • endoplasmic reticulum stress