High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler.
Taras SychJan SchlegelHanna M G BarrigaMiina OjansivuLeo HankeFlorian WeberR Beklem BostanciogluKariem EzzatHerbert StanglBirgit PlochbergerJurga LaurencikieneSamir El AndaloussiDaniel FürthMolly M StevensErdinc SezginPublished in: Nature biotechnology (2023)
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.