FLT3 Amplification as Double Minute Chromosomes in a Patient with Chronic Myelomonocytic Leukemia.
Heyang ZhangXiaoxue WangShibo LiXianfu WangXianglan LuMing LiHua WangYing LiuHui PangLijun ZhangPublished in: Disease markers (2021)
Double minute chromosomes (dmins) are a form of gene amplification presenting as small spherical paired chromatin bodies. Dmins are rare in hematologic malignancies and are generally associated with a poor prognosis. Some case reports identified MYC or MLL gene amplification performing as dmin in myeloid neoplasms. FLT3 (FMS-related tyrosine kinase 3) acts as an oncogene in myeloid neoplasms which is associated with several signal transduction pathways. Genomic amplification of FLT3 has not been reported in hematological disease. The current study attempts to demonstrate the existence of double minute chromosomes via FLT3 gene amplification in a patient diagnosed with chronic myelomonocytic leukemia (CMML). Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient's bone marrow. FLT3 amplification as dmins in a patient with CMML was revealed. This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. The study also proposed another possible mechanism of FLT3 genes in leukemogenesis.
Keyphrases
- acute myeloid leukemia
- tyrosine kinase
- nucleic acid
- copy number
- bone marrow
- case report
- epidermal growth factor receptor
- poor prognosis
- genome wide
- label free
- genome wide identification
- single molecule
- transcription factor
- long non coding rna
- gene expression
- dna methylation
- high resolution
- high throughput
- dna damage
- emergency department
- single cell
- immune response
- oxidative stress
- bioinformatics analysis