The effect of immobilized antioxidant enzymes on the oxidative stress in UV-irradiated rat skin.
Agnieszka Małgorzata PudlarzEwa CzechowskaMichał S KarbownikKatarzyna Ranoszek-SoliwodaEmilia TomaszewskaGrzegorz CelichowskiJarosław GrobelnyEwa ChabielskaAnna Gromotowicz-PopławskaJanusz SzemrajPublished in: Nanomedicine (London, England) (2020)
Aim: Superoxide dismutase (SOD) and catalase (CAT) immobilized on gold nanoparticles (AuNP) and silver nanoparticles (AgNP) nanoparticles were used to reduce UV radiation-induced oxidative stress in rat skin. Materials & methods: The antioxidant influence of the enzymes was investigated on level of malondialdehyde, 8-hydroksy-2'deoksyguanozine, myeloperoxidase, total antioxidant capacity, SOD2 and CAT activity and expression, and glutathione and glutathione peroxidase activity. Results: The application of immobilized SOD and CAT on UV-irradiated skin reduced malondialdehyde and 8-hydroksy-2'deoksyguanozine levels also SOD and CAT activity and expression increased. The tested enzymes influence glutathione peroxidase activity and level of total antioxidant capacity and glutathione. Conclusion: Immobilized enzymes increased the antioxidative potential of skin following UV irradiation.
Keyphrases
- oxidative stress
- gold nanoparticles
- hydrogen peroxide
- soft tissue
- silver nanoparticles
- amyotrophic lateral sclerosis
- poor prognosis
- ionic liquid
- wound healing
- ischemia reperfusion injury
- binding protein
- capillary electrophoresis
- mass spectrometry
- radiation induced
- high resolution
- long non coding rna
- reduced graphene oxide
- atomic force microscopy