Targeted gene deletion with SpCas9 and multiple guide RNAs in Arabidopsis thaliana: four are better than two.

Jana OrdonNiklas KielDieter BeckerCarola KretschmerPaul Schulze-LefertJohannes Stuttmann

Published in: Plant methods (2023)

Multiplex editing with at least two pairs of guide RNAs (four guide RNAs in total) elevates the frequency of chromosomal segment deletions at least at the AtWRKY30 locus, and thus simplifies the selection of corresponding mutants. Co-expression of the TREX2 exonuclease can be used as a general strategy to increase editing efficiency in Arabidopsis without obvious negative effects.

Keyphrases

arabidopsis thaliana
crispr cas
copy number
poor prognosis
high throughput
genome wide
binding protein