Generation of chromosome 1p/19q co-deletion by CRISPR/Cas9-guided genomic editing.

Chao LiZhong LiuXiaoxia ZhangHuafeng WangGregory K FriedmanQiang DingXinyang ZhaoHu LiKitai KimXi YuL Burt NaborsXiaosi HanRui Zhao

Published in: Neuro-oncology advances (2022)

We demonstrated that t(1;19)(q10;p10) may be induced by CRISPR/Cas9-mediated genomic editing. This method represents an important step toward engineering the 1p/19q co-deletion to model oligodendrogliomas. This method may also be generalizable to engineering other cancer-relevant translocations, which may facilitate the understanding of translocation roles in cancer progression.

Keyphrases

crispr cas
genome editing
papillary thyroid
copy number
squamous cell
lymph node metastasis
gene expression
young adults
genome wide