A dual cardiomyocyte reporter model derived from human pluripotent stem cells.
Yuqian JiangXiaoping BaoXiaojun Lance LianPublished in: Stem cell research & therapy (2021)
Cardiovascular diseases (CVD) remain the leading cause of death in the USA. Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. Here, we established a hPSC line integrated with a mCherry fluorescent protein driven by the alpha myosin heavy chain (aMHC) promoter, which could be used to purify CMs based on the aMHC promoter activity in these cells. Combined with a fluorescent voltage indicator, ASAP2f, we achieved a dual reporter CM platform, which enables purification and characterization of CM subtypes and holds great potential for disease modeling and drug discovery of CVD.
Keyphrases
- pluripotent stem cells
- drug discovery
- dna methylation
- cardiovascular disease
- cell therapy
- stem cells
- quantum dots
- gene expression
- induced apoptosis
- binding protein
- living cells
- single cell
- type diabetes
- oxidative stress
- high throughput
- angiotensin ii
- cell death
- cell cycle arrest
- metabolic syndrome
- emergency department
- high glucose
- small molecule
- bone marrow
- human health
- cardiovascular risk factors
- climate change
- electronic health record