Development of a toolkit for piggyBac-mediated integrative transfection of the human filarial parasite Brugia malayi.

Canhui LiuAmruta S MhashilkarJohan ChabanonShulin XuSara LustigmanJohn H AdamsThomas R Unnasch

Published in: PLoS neglected tropical diseases (2018)

The methods and constructs reported here will permit the efficient production of integrated transgenic filarial parasite lines, allowing reverse genetic technologies to be applied to all life cycle stages of the parasite.

Keyphrases

life cycle
plasmodium falciparum
endothelial cells
toxoplasma gondii
trypanosoma cruzi
induced pluripotent stem cells
pluripotent stem cells
gene expression
copy number