Single-base editing of rs12603332 on Chromosome 17q21 with a Cytosine Base Editor regulates ORMDL3 and ATF6α expression.

SNP rs12603332 is functional in regulating the expression of ORMDL3 as well as ORMDL3 regulated gene ATF6α expression. In addition, we demonstrate the use of CBE technology in functionally interrogating asthma-associated SNPs using studies of primary human CD4 cells.