Janus-Type Immunofluorescent Probes and a Quantitative Immunoassay System.
Hiroshi YabuMisako SuzukiKazushige MatsukawaIkuma MaedaSigeo IharaKoshiro YaegashiKentaro TotsuHironori HayashiEiichi KodamaPublished in: Langmuir : the ACS journal of surfaces and colloids (2024)
To realize highly sensitive immunoassays, high optical density probes conjugated with antibodies for target antigens have been demanded in order to increase the visibility of antigen-antibody complex formation. We herein demonstrate the development of an immunoassay system using magnetic and fluorescent Janus particles as probes in conjunction with an antibody-immobilized microfluidic device. The concentration of the detection limit at which there was a significant difference between SARS-CoV-2 and human coronavirus 229E antigens was 3.1 ng/mL, and the standard deviation of the signal was less than 5%. The immunofluorescent probe and immunoassay system developed in this study are expected to be applicable not only to SARS-CoV-2 but also to the quantitative measurement of various other disease marker proteins and biomolecules.
Keyphrases
- sars cov
- living cells
- label free
- fluorescent probe
- small molecule
- single molecule
- respiratory syndrome coronavirus
- high resolution
- sensitive detection
- fluorescence imaging
- endothelial cells
- quantum dots
- dendritic cells
- photodynamic therapy
- high throughput
- molecularly imprinted
- induced pluripotent stem cells
- circulating tumor cells
- high speed
- pluripotent stem cells
- tandem mass spectrometry
- mass spectrometry