Mass-cytometry-based quantitation of global histone post-translational modifications at single-cell resolution across peripheral immune cells in IBD.
Lawrence BaiDenis DermadiLaurynas KalesinskasMai DvorakSarah E ChangAnanthakrishnan GanesanSamuel J S RubinAlex KuoPeggie CheungMichele DonatoPaul J UtzAida HabtezionPurvesh KhatriPublished in: Journal of Crohn's & colitis (2022)
We describe the first systems-level quantification of histone modifications across immune cells from IBD patients at a single-cell resolution revealing the increased epigenetic heterogeneity that is not possible with traditional ChIP-seq profiling. Our data open new directions in investigating the association between histone modifications and IBD pathology using other epigenomic tools.
Keyphrases
- single cell
- dna methylation
- rna seq
- high throughput
- ulcerative colitis
- genome wide
- gene expression
- single molecule
- ms ms
- minimally invasive
- mass spectrometry
- electronic health record
- liquid chromatography tandem mass spectrometry
- circulating tumor cells
- big data
- tandem mass spectrometry
- artificial intelligence
- data analysis