Targeted Detoxification of Aflatoxin B 1 in Edible Oil by an Enzyme-Metal Nanoreactor.

Mycotoxin contamination is an important issue for food safety and the environment. Removing mycotoxins from food without losing nutrients and flavor components remains a challenge. In this study, a novel strategy was proposed for the targeted removal of aflatoxin B 1 (AFB 1 ) from peanut oil using an amphipathic enzyme-metal hybrid nanoreactor (PL-GOx-Fe 3 O 4 @COF) constructed with covalent organic frameworks (COFs) which can selectively adsorb AFB 1 . Due to the confined space provided by COFs and the proximity effect between GOx and Fe 3 O 4 , the detoxification of AFB 1 is limited in the nanoreactor without affecting the composition and properties of the oil. The detoxification efficiency of AFB 1 in the chemoenzymatic cascade reaction catalyzed by PL-GOx-Fe 3 O 4 @COF is six times higher than that of the combination of free GOx and Fe 3 O 4 . The AFB 1 transformation product has nontoxicity to kidney and liver cells. This study provides a powerful tool for the targeted removal of mycotoxins from edible oils.