Structural and functional characterization of the bacterial biofilm activator RemA.
Tamara HoffmannDevid MrusekPatricia BedrunkaFabiana BurchertChristopher-Nils MaisDaniel B KearnsFlorian AltegoerErhard BremerGert BangePublished in: Nature communications (2021)
Bacillus subtilis can form structurally complex biofilms on solid or liquid surfaces, which requires expression of genes for matrix production. The transcription of these genes is activated by regulatory protein RemA, which binds to poorly conserved, repetitive DNA regions but lacks obvious DNA-binding motifs or domains. Here, we present the structure of the RemA homologue from Geobacillus thermodenitrificans, showing a unique octameric ring with the potential to form a 16-meric superstructure. These results, together with further biochemical and in vivo characterization of B. subtilis RemA, suggests that the protein can wrap DNA around its ring-like structure through a LytTR-related domain.
Keyphrases
- transcription factor
- dna binding
- bacillus subtilis
- genome wide identification
- circulating tumor
- candida albicans
- cell free
- binding protein
- genome wide
- single molecule
- biofilm formation
- poor prognosis
- protein protein
- staphylococcus aureus
- bioinformatics analysis
- pseudomonas aeruginosa
- amino acid
- high frequency
- gene expression
- ionic liquid
- small molecule
- escherichia coli
- toll like receptor
- risk assessment
- inflammatory response